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KMID : 1134820200490070774
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 7 p.774 ~ p.780
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Optimization of Preparation Conditions for Liposome Containing Ginsenoside Re by Response Surface Methodology
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Chang Hyeon-Ho
Lee Eui-Seok
Bae Bong-Seok
Hong Soon-Taek
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Abstract
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This experiment was carried out to study the process and optimization conditions for the preparation of ‘ginsenoside Re liposome’ using response surface methodology. Ginsenoside Re fractions obtained from ginseng berry, were incorporated in the lipid membrane of phosphatidylcholine (PC), and subjected to a homogenization process to prepare ginsenoside Re liposomes. In the experimental design, the independent variables were the amount of PC, a raw material for producing liposome, and the ginsenoside Re, the loading material. The dependent variables were the loading efficiency, and the loaded amount based on the PC unit concentration. As a result, the maximum loading efficiency was observed in the treatment of the highest PC and the lowest ginsenoside Re formulation (No. 2 treatment) (approximately 71%), while the maximum load per PC unit concentration was in the treatment of the lowest PC and the highest ginsenoside Re formulation (No 3 treatment) (about 14 mg/g PC). The optimization condition was determined by overlaying the change of the loading efficiency and the loaded amount according to the combination of each independent variable. The optimal combination of PC and ginsenoside Re was 3.11925 g and 42.51 mg, respectively, and the loading efficiency and the loaded amount per PC unit concentration were predicted to be 58.7114% and 7.93069 mg/g PC, respectively.
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KEYWORD
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liposome, ginsenoside Re, response surface methodology, homogenization
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